Expression analysis of JAZ family genes in harvested cucumber fruit under cold storage con-dition
Chilling injury poses a significant challenge to the postharvest storage and transportation of cucumber fruits.Jasmonic acid(JA)plays a crucial role in regulating the chilling resistance in plants.JAZ(JA ZIM-domain)proteins act as transcription repressors of JA signaling,inhibiting both the synthesis and transmission of JA.To ex-plore the role of JAZ family genes during cold storage of harvested cucumber fruits,JAZ family genes were identified and screened from the cucumber genome,and their sequence features,physicochemical properties,and spatial-tem-poral expression patterns were analyzed.The findings revealed a total of 17 JAZ genes in the cucumber genome,which could be divided into 8 subfamilies,all containing the conserved Tify or CCT domains in their protein se-quences.Most of these JAZ genes responded to cold storage(5 ℃ and 10℃)treatments,suggesting the involve-ment of JAZ family proteins in regulating the chilling tolerance in cucumber fruits.However,different JAZ genes ex-hibited varying degrees of response to cold treatments.The promoters of cucumber JAZ family genes had numerous stress-responsive elements,though the specific types of elements varied.During 5 ℃ storage period,the expression levels of 14 JAZ genes gradually decreased,while the expression of JAZ11 significantly increased with storage time.Exogenous treatments with abscisic acid(ABA),methyl jasmonate(MeJA),melatonin(MT),as well as the silen-cing HHO2 and GRP3 expression in harvested cucumbers,significantly impacted the expression of JAZ family genes.These results further confirmed the involvement of cucumber JAZ family genes in regulating chilling tolerance.ABA,MeJA,and MT treatments significantly downregulated JAZ11 expression,whereas silencing HHO2 and GRP3 signifi-cantly upregulated its expression.These results together suggest that JAZ11 negatively regulates J A signaling and cold tolerance in cucumber fruits.This study provides novel insights into the physicochemical properties and expression patterns of cucumber JAZ family genes under cold conditions,and suggesting that JAZ11 may be the key factor re-sponsible for negative regulation of chilling tolerance in harvested cucumbers among JAZ family proteins.
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