首页|耐草甘膦和啶嘧磺隆的转基因水稻研究

耐草甘膦和啶嘧磺隆的转基因水稻研究

扫码查看
杂草防治是水稻生产中的重要环节.该研究将耐草甘膦基因CP4-EPSPS和耐除草剂基因P450-N-Z1转入粳稻品种秀水134中,筛选获得一个对草甘膦以及啶嘧磺隆均具有良好耐受性的转化体GF-9.田间试验结果显示,该转化体能够耐受1 800 g·hm-2(以酸当量计)草甘膦酸和30 g·hm-2(以有效成分计)啶嘧磺隆活性成分的复合除草剂.分子特征鉴定结果表明,GF-9是单拷贝T-DNA转化体,其整合位点不在已知的注释基因中.田间初步试验表明,GF-9的田间表现型与非转基因对照相比无显著差异.综上所述,GF-9是具有潜在商业价值的转基因耐除草剂水稻转化体.
A transgenic rice resistant to glyphosate and flazasulfuron
Weed control is an important aspect of rice production.In this study,a transgenic herbicide tolerant rice was created by expressing the glyphosate tolerant gene CP4-EPSPS and multiple-herbicide tolerant gene P450-N-Z1.The transformant event GF-9 was selected by screening with two herbicides,glyphosate and flazasulfuron.Field trial demonstrated that theGF-9 could tolerate combined herbicides at rate of 1 800 g·hm-2(based on acid equivalent)glyphosate and 30 g·hm-2(based on active ingredients)flazasulfuron.Southern blot and high-efficiency thermal asymmetric interlaced PCR results showed that the GF-9 was a single copy T-DNA insertion event and its insertion site was not inside a known or predicted functional gene.The field agronomic performance of GF-9 showed no signifi-cant difference compared to non-transgenic controls.In summary,the transgenic herbicide tolerant event GF-9 is a potential commercial event for development of herbicide tolerant rice.

ricetransgenicherbicide tolerant

吴浩峰、林朝阳、沈志成

展开 >

浙江大学昆虫科学研究所,浙江 杭州 310058

浙江大学新农村发展研究院,浙江 杭州 310058

水稻 转基因 耐除草剂

浙江省"领雁"研发攻关计划

2023C02033

2024

浙江农业学报
浙江省农业科学院 浙江省农学会

浙江农业学报

CSTPCD北大核心
影响因子:0.765
ISSN:1004-1524
年,卷(期):2024.36(9)