Weed control is an important aspect of rice production.In this study,a transgenic herbicide tolerant rice was created by expressing the glyphosate tolerant gene CP4-EPSPS and multiple-herbicide tolerant gene P450-N-Z1.The transformant event GF-9 was selected by screening with two herbicides,glyphosate and flazasulfuron.Field trial demonstrated that theGF-9 could tolerate combined herbicides at rate of 1 800 g·hm-2(based on acid equivalent)glyphosate and 30 g·hm-2(based on active ingredients)flazasulfuron.Southern blot and high-efficiency thermal asymmetric interlaced PCR results showed that the GF-9 was a single copy T-DNA insertion event and its insertion site was not inside a known or predicted functional gene.The field agronomic performance of GF-9 showed no signifi-cant difference compared to non-transgenic controls.In summary,the transgenic herbicide tolerant event GF-9 is a potential commercial event for development of herbicide tolerant rice.
维普
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