A transgenic rice resistant to glyphosate and flazasulfuron
Weed control is an important aspect of rice production.In this study,a transgenic herbicide tolerant rice was created by expressing the glyphosate tolerant gene CP4-EPSPS and multiple-herbicide tolerant gene P450-N-Z1.The transformant event GF-9 was selected by screening with two herbicides,glyphosate and flazasulfuron.Field trial demonstrated that theGF-9 could tolerate combined herbicides at rate of 1 800 g·hm-2(based on acid equivalent)glyphosate and 30 g·hm-2(based on active ingredients)flazasulfuron.Southern blot and high-efficiency thermal asymmetric interlaced PCR results showed that the GF-9 was a single copy T-DNA insertion event and its insertion site was not inside a known or predicted functional gene.The field agronomic performance of GF-9 showed no signifi-cant difference compared to non-transgenic controls.In summary,the transgenic herbicide tolerant event GF-9 is a potential commercial event for development of herbicide tolerant rice.