Analysis of the B3 transcription factor alternative splicer in foxtail millet(Setaria italica L.)
The alternative splicing types of B3 transcriptional factor gene in different millet varieties were analyzed to i-dentify the splicing variants with functional activity.Furthermore,the correlation between these splicing variants and heading stage was analyzed,aiming at providing evidence for further revealing the regulatory effects of B3 transcription factor on growth and development of millet by alternative splicing.Totally 12 millet varieties with different heading sta-ges were selected to clone B3 transcriptional factor gene belonging to REM family by reverse transcription PCR(RT-PCR).The alternative splicing types of this gene were identified by multiple sequence alignment,and the activity of the splicing variants,their association with heading stage were analyzed.The results showed that a total of 8 splicing va-riants of B3 transcriptional factor gene were produced among the 12 millet varieties,of which splicing variant Ⅴ contained the most varieties(4),followed by splicing variant Ⅳ(2),and the remaining 6 splicing variants each contained only 1 variety.By alternative splicing,the B3 transcriptional factor gene of 12 varieties encoded 10 types of proteins,of which 5 types lacked the B3 DNA binding domain,and type Ⅱ protein shortened the heading stage of millet,while type Ⅵ protein prolonged the heading stage of millet,which was not affected by geographical environments.The above results showed that B3 transcription factor participated in the regulation of heading stage through alternative splicing in millet.
万方数据
维普
