In order to understand the genetic diversity of hulless barley germplasms from different regions and to dis-cover markers associated with agronomic traits.In this study,we analyzed the genetic diversity and population genet-ic structure of 102 hulless barley germplasms from different regions of Gansu and Xizang using 92 pairs of SSR mark-ers,and the association analysis between SSR markers and agronomic traits was carried out by using the GLM model of Tassel 2.1 software.The results showed that a total of 739 alleles were identified by scanning 92 markers,and the variance of gene diversity index ranged from 0.106 to 0.913,the variance of polymorphism information content ranged from 0.103 to 0.907;the genetic similarity coefficient varied from 0.680 to 0.960,and the clustering analy-sis divided these hulless barley germplasms into two subgroups,germplasms with the same parentage or similar kin-ship were clustered together;the population genetic structure analysis divided the hulless barley germplasms into five subgroups;it was revealed that more than 9 agronomic traits of 33 markers were extremely remarkalbe at the P<0.01 level,there were 10 markers associated with thousand grain weight,of which the marker GBM1238 was more stable,and the explained rate of the phenotypic variance of these markers ranged from 8.95%to 35.02%.In conclusion,most of the hulless barley germplasms for the test in this study are similarly related,and different hulless barley ge-netic resources can be introduced to enrich the genetic background.