摘要
选取同一批次14、28、42、70、660日龄的母番鸭屠宰后采集胸肌和腿肌,测定水分、蛋白质和氨基酸含量的变化,旨在明确番鸭胸肌和腿肌氨基酸谱的发育性变化.结果表明,在整个发育过程中,番鸭的胸肌重、腿肌重均随日龄增长而显著(P＜0.05)增加,蛋白含量总体增加,水分含量逐渐降低.番鸭胸肌的氨基酸总量和必需氨基酸含量随日龄增长总体增加,70日龄与660日龄间无显著差异,但显著高于14日龄、28日龄、42日龄,赖氨酸和胱氨酸含量在70日龄时显著高于其他日龄,精氨酸、酪氨酸、缬氨酸、苯丙氨酸、异亮氨酸和脯氨酸的含量在660日龄显著高于其他日龄.番鸭腿肌的氨基酸总量、必需氨基酸含量和鲜味氨基酸含量均在70日龄时达到峰值,丙氨酸、亮氨酸和赖氨酸的含量在70日龄显著高于其他日龄,胱氨酸、苏氨酸、酪氨酸的含量在660日龄显著高于其他日龄.正交偏最小二乘判别分析模型表明,不同日龄番鸭的胸肌和腿肌氨基酸谱存在差异,赖氨酸和胱氨酸分别是14～660日龄番鸭胸肌和腿肌氨基酸代谢谱差异中最重要的氨基酸.综上,番鸭肌肉中的氨基酸含量随着日龄的增长而发生明显变化,腿肌中的总氨基酸、必需氨基酸和鲜味氨基酸含量在70日龄较为突出,胸肌中的总氨基酸和必需氨基酸含量在660日龄尤为丰富.
Abstract
Samples of breast and leg muscles from the same batch of female Muscovy ducks at 14,28,42,70,and 660 days of age were collected post-slaughter,and the changes in moisture,protein,and amino acid content were de-termined,to clarify the developmental changes in amino acid profiles of Muscovy duck breast and leg muscles.Re-sults indicated that both breast and leg muscle weights increased significantly(P＜0.05)with age,while the protein content increased overall yet the moisture content gradually decreased during the entire developmental process.The total amount of amino acids and essential amino acids in breast muscles increased with age,showing no significant difference between 70 and 660 days but significantly higher levels compared to 14,28,and 42 days.Lysine and cys-teine levels were significantly higher at 70 days,while arginine,tyrosine,valine,phenylalanine,isoleucine,and proline levels were significantly higher at 660 days.In leg muscles,total amino acid content,essential amino acids,and umami amino acids peaked at 70 days,with significantly higher levels of alanine,leucine,and lysine compared to other ages.Cysteine,threonine,and tyrosine levels were significantly higher at 660 days.Orthogonal partial least squares discriminant analysis revealed differences in amino acid profiles between breast and leg muscles of Muscovy ducks at different ages,with lysine and cysteine identified as the most important amino acids contributing to metabol-ic profile differences from 14 to 660 days.In conclusion,amino acid content in Muscovy duck muscles undergoes significant changes with age,with notable peaks in total amino acids,essential amino acids,and umami amino acids in leg muscles at 70 days,and in total amino acids and essential amino acids in breast muscles at 660 days.
基金项目
国家现代农业产业技术体系水禽体系专项资金(CARS-42-27)
维普
万方数据