With the continuous expansion of transgenic crop planting area,how to efficiently deal with the transgenic straw has become an important scientific issue.The recombinant protein and recombinant DNA in untreated transgen-ic straw can persist in the soil for a long time and have a potentially negative impact on soil biodiversity.Therefore,it is of importance to find a cost-saving and environmentally friendly straw treatment method.High temperature treat-ment is an effective method to degrade the recombinant protein and recombinant DNA of transgenic straw,but the treatment temperature and treatment time have not been systematically studied at present.This study used the test strips and polymerase chain reaction(PCR)to detect the levels of recombinant protein and recombinant DNA in the transgenic straw under different temperature and time treatments,and found that phosphinothricin acetyltransferase(PAT)and other recombinant proteins in the straw of transgenic soybeans,cotton,maize and rice were almost de-graded after being treated at 50 ℃ for 3 h.However,at the same temperature,the degradation of recombinant DNA took 4 d.The degradation time of recombinant proteins and recombinant DNA could be shorted by increasing the tem-perature.These combinations of temperature and time,particularly the treatment condition of 50 ℃ for 4 d,could be achieved by composting in agricultural production.Therefore,this study provided a theoretical basis for the treatment of transgenic straw from the perspective of molecular biology.
维普
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