Pine-seed oil was extracted from pine-seed cake by aqueous enzymatic method.The extraction process of pine seed oil was investigated using single-factor and response surface experiments,and a specific focus on demulsif-ying the emulsion formed during the extraction.The results showed that the best outcomes were achieved through al-kaline protease hydrolysis.The optimum conditions were as follows:time of 2.5 h,temperature 56 ℃,pH value of 11.3,and enzyme dosage of 5 045 U·g-1.Under these conditions,the extraction rate of pine seed oil was 71.73％.Furthermore,the addition of 3 000 U·g-1 of alkaline protease resulted in the highest demulsification effect,with a demulsification rate of 87.68％.After enzymatic demulsification,the demulsification rate was further improved to 92.18％by treating the emulsion with acid to adjust the pH value to 5.As a result,the extraction rate of pine seed oil reached 70.36％under this condition.
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