Effects of enzyme-assisted extraction on composition and gut microbiota regulation function of Dendrobium officinale polysaccharide
Enzymatic hydrolysis may alter the number or structure of certain groups within the polysaccharide mole-cule,thus altering its properties.To determine the effect of enzymatic hydrolysis on the structure and function of Dendrobium officinale polysaccharide,this study selected the same species of D.officinale fresh stems as raw materi-als,and obtained different structures of the same matrix polysaccharide through neutral protease,maltose amylase,and cellulase-assisted extraction.Then,analyzing structural differences between them,and studying their various regulatory functions to intestinal flora using an in vitro simulated fermentation system.The results showed that com-pared with unenzymatically hydrolyzed samples,neutral protease-assisted extraction increased the degree of polymeri-zation of polysaccharides,while the intervention of cellulase and maltose amylase significantly reduced the degree of polysaccharide polymerization and changed their monosaccharide composition.The production of CH4 and H2S in the neutral protease-treated group increased significantly,while the production of butyric acid and valeric acid in the cel-lulase-treated group increased significantly.Polysaccharides improved the composition of intestinal flora,especially the cellulase-treated group.The types and relative abundance of intestinal cornerstone bacteria as well as beneficial bacteria,such as Bifidobacterium,Lactobacillus,Faecalibacterium and Roseburia,increased significantly,while the relative abundance of opportunistic pathogenic bacteria,such as Dialister and Dorea,decreased significantly.There were nine bacterial genera that were significantly positively correlated with butyric acid and valeric acid levels,and eight of them were enriched in the cellulase-treated group.Enzymatic hydrolysis-assisted extraction changes the struc-ture of D.officinale polysaccharides,thereby changing its regulatory functions to intestinal flora.This study provides a reference for the targeted production of polysaccharides with different functions.
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维普
