摘要
为利用电感耦合等离子体串联质谱法(ICP-MS/MS)建立同时测定三叶青中Na、Mg、Al、P、S、K、Ca、Fe、Ni、Cu、Zn、Se共12种无机营养元素含量的方法,并探究不同部位(茎叶、块根、须根)的12种元素含量分布,通过ICP-MS/MS中的单杆和串联四级杆模式下的多种反应气体(02、H2、NH3)形式建立同时分析三叶青中12种营养元素的方法,测定三叶青中不同部位的无机营养元素含量,运用单因素方差分析(ANOVA)比较药材不同部位无机营养元素含量的差异,采用偏最小二乘-判别分析(PLS-DA)构建不同部位的鉴别模型.结果表明,ICP-MS/MS方法的线性、检测限、精密度、重复性和稳定性均良好,实际药材的加样回收率为90.66％～108.50％;不同部位的三叶青无机营养元素含量差异较大,地下部的无机营养元素含量显著高于地上部,Ca、Al、K、Na这4种元素是三叶青3个部位的主要差异元素.本研究通过电感耦合等离子体串联质谱法建立了快速鉴别三叶青不同部位中无机营养元素含量的方法,有效鉴定了块根、须根、茎叶中的无机营养元素含量,三叶青地下部的无机营养元素含量显著高于地上部,研究结果可以为三叶青质量控制、原产地溯源和种质资源的评价提供技术依据.
Abstract
To establish a method for the simultaneous determination of 12 inorganic nutrient elements(Na,Mg,Al,P,S,K,Ca,Fe,Ni,Cu,Zn,Se)in Tetrastigma hemsleyanum Diels et Gilg(T.hemsleyanum)by inductively coupled plasma tandem mass spectrometry(ICP-MS/MS),and to explore the content distribution of these 12 inor-ganic nutrient elements in different parts(stem and leaf,tuberous root,fibrous root).The method for simultaneous analysis of 12 nutrient elements in T.hemsleyanum was established by ICP-MS/MS in the form of multiple reaction gases(02,H2,NH3)under the mode of single rod and series four rod,and the contents of inorganic nutrient ele-ments in different parts of T.hemsleyanum were determined,and the differences of inorganic nutrient elements in dif-ferent parts of medicinal materials were compared by one-way analysis of variance(ANOVA).Partial least squares-discriminant analysis(PLS-DA)was used to construct identification models for different parts.The results showed that the linearity,detection limit,precision,repeatability and stability test results of ICP-MS/MS method were good,and the recoveries ranged from 90.66％to 108.50％.There were significant differences in contents of inorganic nutrient el-ements in different parts of T.hemsleyanum,and the contents of inorganic nutrients in root were significantly higher than those in aboveground.Ca,Al,K and Na were the main difference elements among the three parts of T.hemsleya-num.In this study,the rapid analysis and determination of T.hemsleyanum inorganic elements was established,and the contents of inorganic elements in tuberous root,fibrous root,stem and leaf were effectively identified,which pro-vided technical basis for quality control,origin tracing and germplasm resource evaluation of T.hemsleyanum.
基金项目
浙江省基础公益研究计划(LGN22H280010)
浙江省自然科学基金(LY21H280008)
国家自然科学基金(82304654)
维普
万方数据