ICP-MS/MS analysis and evaluation of inorganic nutrient elements in different parts of Tet-rastigma hemsleyanum Diels et Gilg
To establish a method for the simultaneous determination of 12 inorganic nutrient elements(Na,Mg,Al,P,S,K,Ca,Fe,Ni,Cu,Zn,Se)in Tetrastigma hemsleyanum Diels et Gilg(T.hemsleyanum)by inductively coupled plasma tandem mass spectrometry(ICP-MS/MS),and to explore the content distribution of these 12 inor-ganic nutrient elements in different parts(stem and leaf,tuberous root,fibrous root).The method for simultaneous analysis of 12 nutrient elements in T.hemsleyanum was established by ICP-MS/MS in the form of multiple reaction gases(02,H2,NH3)under the mode of single rod and series four rod,and the contents of inorganic nutrient ele-ments in different parts of T.hemsleyanum were determined,and the differences of inorganic nutrient elements in dif-ferent parts of medicinal materials were compared by one-way analysis of variance(ANOVA).Partial least squares-discriminant analysis(PLS-DA)was used to construct identification models for different parts.The results showed that the linearity,detection limit,precision,repeatability and stability test results of ICP-MS/MS method were good,and the recoveries ranged from 90.66％to 108.50％.There were significant differences in contents of inorganic nutrient el-ements in different parts of T.hemsleyanum,and the contents of inorganic nutrients in root were significantly higher than those in aboveground.Ca,Al,K and Na were the main difference elements among the three parts of T.hemsleya-num.In this study,the rapid analysis and determination of T.hemsleyanum inorganic elements was established,and the contents of inorganic elements in tuberous root,fibrous root,stem and leaf were effectively identified,which pro-vided technical basis for quality control,origin tracing and germplasm resource evaluation of T.hemsleyanum.
Tetrastigma hemsleyanum Diels et Gilginorganic elementinductively coupled plasma tandem mass spectrometrypartial least squares discriminant analysis
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