Genomic-wide identification and expression analysis of the PP2C gene family in sweet cherry
Protein phosphatase (protein phosphatase 2C,PP2C),as a key regulator of ABA signaling pathway,plays an important role in plant growth,hormone transduction and stress response.To identify and analyze the re-sponse of sweet cherry PP2C gene family to different abiotic stresses,the stress resistance function of this family members were explored in sweet cherry,in order to provide a theoretical basis for improving their stress resistance by genetic engineering technology.The whole genome file was downloaded from Rosaceae genome database (https∶//www.rosaceae.org/) .Bioinformatics was used to study the physicochemical properties,genetic relationships,gene structure,chromosome localization and collinearity,promoter cis-elements,etc.Cherry Gisela No.7 test-tube seed-lings were used as materials,real-time fluorescence quantification PCR (qRT-PCR) was used to analyze the expres-sion patterns.A total of 76 members of the sweet Cherry PP2C family were identified,whose protein sequence length ranged from 125 to 1580 amino acids,theoretical isoelectric point (pI) ranged from 4.57 to 9.40,and 72％ of the members were acidic proteins.Chromosomal localization showed that they were unevenly distributed on 8 chromo-somes of sweet cherry,including 14 collinear gene pairs,which could be divided into 12 subfamilies.Subcellular lo-calization prediction showed that the family members were mainly distributed in the nucleus.Additionally,Analysis of cis-acting elements displayed that the upstream 2000 bp promoter region of 76 PaPP2C genes contained multiple elements,such as elements of hormone,light and environmental stress.The results of qRT-PCR analysis of 10 genes showed that PaPP2C23,PaPP2C47,PaPP2C48 and PaPP2C49 genes were significantly up-regulated under drought (PEG),salt (NaCl) and abscisic acid (ABA) stresses,which was speculated that the above genes could regulate drought and salt stress by relying on abscisic acid.Additionally,PaPP2C71,PaPP2C5,PaPP2C49,PaPP2C23,PaPP2C59,PaPP2C60 and PaPP2C74 were significantly down-regulated,indicating that they might play negative regulatory role in low temperature stress.Sweet cherry PP2C family members can play important role in biotic stresses such as drought,salt,low temperature,and abscisic acid.
sweet cherry 2C protein phosphatasedroughtmicrothermcis-acting element
万方数据
维普
