Expression patterns and transcriptional autoactivation analysis of CaERF70 in chili pepper
The AP2/ERF transcription factor family is widely involved in biological processes such as plant growth and development and stress response,so it is of great significance for the study of AP2/ERF transcription factors.In this study,we cloned a ERF gene,CaERF70 from salt-tolerant variety SHA2022119 of pepper,which contains 819 bp,encoding 272 amino acids,molecular weight of about 30.59 ku,and isoelectric point pI of 7.67.Bioinformatics analysis showed that the protein had a character with negatively charged,unstable,34 potential phosphorylation sites,non-transmembrane and expressed in the nucleus.The phylogenetic tree results indicated that CaERF70 was clustered with the ERFs of tomato,tobacco and potato,suggesting that CaERF70 was very conserved in the evolution of Solanaceae.qRT-PCR showed that the expression of CaERF70 gene was induced by low temperature and sup-pressed by high temperature.Prokaryotic expression experiments showed that the size of the protein encoded by CaE-RF70 was about 30 ku consistent with the predicted size.Transcriptional activation activity analysis showed that CaE-RF70 had transcriptional activation activity.Taken together,the above results indicated that CaERF70 was an AP2/ERF2 transcription factor,belonging to the ERF Ⅶ group,with transcriptional activating activity,and the expression of CaERF70 was significantly changed due to high or low temperature stress,suggesting that CaERF70 was involved in the response of pepper to high or low temperature stress.
万方数据
维普
