Determination of antifungal spectrum and evaluation of antifungal activity of Trichoderma viride B3 strain
In order to explore the antifungal spectrum and antifungal activity of Trichoderma viride B3 strain,the con-frontation culture and microscopic observation were used to determine and clarify the antifungal spectrum and mecha-nism of T.viride B3 strain.The results showed that T.viride B3 strain had antagonistic effects against 8 plant patho-genic fungi.It had the strongest inhibition effect on the colony growth of Monilia spp.and Botrytis cinerea among the 8 plant pathogenic fungi,with the growth inhibition rates of 90.8％ and 88.9％,respectively,and the antagonistic level went to Grade I;while it had the weakest inhibition effect on the growth of Alternaria mali,with the inhibition rate of 48.2％ and the antagonistic level went to Grade Ⅲ.The observation of confrontation culture showed that,when T.viride B3 strain and Monilia spp.contacted with each other,T.viride B3 strain quickly occupied the nutri-tional growth space of the pathogenic fungi,the contact between colonies and pathogen colonies made pathogen hyphae entangled,expanded,contracted and dissolved.In conclusion,T.viride B3 strain had antagonistic effect a-gainst Monilia sp.and B.cinerea.
万方数据
维普
