Identification of Lycium barbarum root rot disease pathogens and biocontrol funguses against root rot disease
Root rot disease of Lycium barbarum is a common soil-borne disease that occurs in L.barbarum cultivation areas.It is caused by a complex infection of multiple pathogenic fungi and is currently primarily controlled through chemical means.The aim of this study is to explore the feasibility of rapid screening of highly effective,broad-spec-trum and practical biocontrol fungi with disease prevention and growth promotion effects,and identify the main patho-genic bacteria of L.barbarum root rot in Ningxia region and screen excellent antagonistic strains.Using the diseased root of Ningqi No.1 L.barbarum and the rhizosphere soil as materials,pathogenic funguses and rhizosphere microor-ganisms were isolated and purified using tissue separation method;the inhibitory effect of rhizosphere microorganisms on isolated pathogenic funguses was determined using the confrontation method;the antagonistic fungi was identified based on colony culture,morphological characteristics,and rDNA-ITS sequence.The results showed that the main strains isolated from the diseased root tissues of L.barbarum were Fusarium oxysporum,Fusarium solani,Fusarium dimrum,Fusarium equiseti,Fusarium petroliphilum,and Mortierella alpina,with frequencies of 29.5％,16.8％,13.7％,8.9％,8.9％,and 7.4％,respectively.Aspergillus terreus and Trichoderma longibrachiatum have obvi-ous inhibition effect on the main pathogenic funguses,with the inhibition rate of more than 37％,which can effec-tively adjust the microbial flora of the root zone of L.barbarum and promote the restoration and balance of soil mi-cro-ecology.
万方数据
维普
