Preparation and antioxidant activity of propolis oil extracts
Propolis is a good medicinal and edible natural product,which have a wide range of biological activities.The purpose of this study was to explore the effect of camellia oil extraction on the active components and antioxidant activity in propolis.Firstly,total flavonoids were used as the evaluation index,this study obtained the optimal process conditions for propolis extraction by one-way and response surface experiments.Then,we evaluated the an-tioxidant activity of the oil extracts including DPPH scavenging ability and ferric iron reduction ability.Finally,the mechanism of the antioxidant activity of galangin,a typical fat-soluble compound in propolis,was investigated using molecular docking was explored.The results showed that the optimal process was as follows∶extraction temperature 41 ℃,liquid-solid ratio 16∶1 mL·g-1,extraction time 31 h,at which the content of total flavonoids was 35.59 mg·g-1 .The IC50 value of the oil extracts obtained under this process was 12.48 μg·mL-1 for DPPH scavenging,and the total antioxidant capacity(FRAP) was 3.70 mmol·g-1 .In addition,the molecular docking results indicated that galangin,a typical fat-soluble component of propolis,was closely related to the antioxidant effect of propolis.
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