Research progress on the structure and assembly of plant supramolecular cellulose synthase complexes
Cellulose is the main component of plant cell wall and the most abundant renewable resource in nature.Plant cellulose is composed of β-1,4-glucan chains,and its synthesis is catalyzed by the cellulose synthase complex (CSC) located on the plasma membrane.In this paper,the latest research findings on the structure and assembly of plant CSC were analyzed from the perspectives of biochemistry,genetics and evolution,these included examining the protein structure characteristics and biological functions of cellulose synthase (CesA) subunits,the mechanism by which CesAs assembly into CSC rosettes core complex,and the relationship between CSC structure and cellulose properties.The roles of auxiliary proteins such as cytoskeleton and post-translational modification of proteins in the as-sembly of supramolecular CSC and the regulation of cellulose synthesis was further explored.This paper prospected the use of single base editing technology to mutate key amino acid sites in domains such as plant-conserved region (PCR),N-terminal domain (NTD) or transmembrane domain (TM) .By regulating the stability and function of CSC,it is ex-pected to cultivate new plants with increased biomass,enhanced stress resistance and suitable for specific industrial ap-plications,which provides a novel strategy for genetic improvement of plant cellulose genetic engineering.
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维普
