Exploring ADSL gene regulation of inosine monophosphate content in Bashang long tail chicken based on transcriptome and next-generation sequencing technology
In order to search and improve the genes involved in the muscle inosine monophosphate(IMP)content of Bashang long tail chickens,a high-quality meat and egg dual-use breed of Bashang long tail chickens in Hebei Prov-ince was selected as the experimental subjects.The inosine content of 60 chickens was measured.The high and low inosine monophosphate content of Bashang long tail chickens was used as the indicator for grouping,and transcrip-tome sequencing was performed for differential expression gene analysis and KEGG pathway enrichment.PCR ampli-fication and sequencing technology were used to screen for polymorphic sites.Use one-way ANOVA in SPSS to con-duct correlation analysis with inosine monophosphate content.The results showed that:among the significantly differ-entially expressed genes in transcriptome sequencing analysis,there were 251 up-regulated genes and 241 down-regu-lated genes,while 18 300 genes were not significantly different.KEGG pathway enrichment analysis was conducted on the differentially expressed genes,and it was found that the nucleotide metabolism pathway has an important im-pact on the content of inosine monophosphate.The ADSL gene is involved in this pathway and ultimately affects the synthesis of inosine monophosphate.the 2nd,7th,9th,and 11th exons of the ADSL gene were successfully ampli-fied,and g.3713G＞A,g.3797C＞T,g.8282C＞G,g.10191 C＞T,and g.12827C＞A were screened.Among them,the IMP content in exon 2(g.3797C＞T)CT individuals was significantly(P＜0.05)higher than that in CC and TT individuals,while in exon 7(g.8282C＞G),the IMP content in CG individuals was significantly(P＜0.05)higher than that in GG individuals and CC individuals.The dominant genotypes of the ADSL gene in Bashang long tail chickens are located at exon 2,g.3797C＞T site CT type,and exon 7,g.828282C＞G site CG type,which can be used as molecular markers for IMP content in breeding practice.
Bashang long tail chickentranscriptome sequencingADSLpolymorphic locusinosine monophos-phate
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