To investigate the effect of gypenoside on bovine viral diarrhea virus(BVDV),the in vitro cell culture technique combined with cytopathic effect(CPE)observation and CCK-8 assay was employed.The safe concentra-tions of gypenoside and α-glycerol monolaurate were determined.Three different treatment protocols were used:pre-treatment with compound followed by viral infection,post-treatment with compound after viral infection,and pre-in-cubation of compound and virus prior to infection.Western blot and quantitative PCR were conducted to evaluate the changes of expression levels of BVDV E2 protein gene and 5'-UTR,to explore the inhibitory effect in vitro.The re-sults showed that the cell viability at the concentration of 16 μmol·L-1 for gypenoside and 25 μg·mL-1 for α-glycerol monolaurate was not significantly different from the control group(P＞0.05),indicating that these concentrations were the maximum safe concentrations of the two compounds.Compared to the control group,gypenoside exhibited significant(P＜0.01)inhibition on the expression levels of BVDV E2 protein gene and 5'-UTR,and its direct viru-cidal effect was superior to the α-glycerol monolaurate.Gypenoside also showed significant(P＜0.05)adsorption inhibition against BVDV,while no significant difference was found in replication inhibition when compared to the vi-rus control group.
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