Identification and biological characteristics of phytopathogen in Osmanthus fragrans in Zhe-jiang Province of China
Osmanthus fragrans,an evergreen tree with tremendous economic and ornamental value,has been exten-sively planted around the world.However,the disease of leaf spot seriously affected the quality and safety of Osman-thus fragrans due to uniform variety cultivation in Hangzhou.In this study,we isolated one strain that caused leaf spots and identified the fungi as Botryosphaeria dothidea by ITS sequence analysis.To clarify different factors that in-fluence the growth of Botryosphaeria dothidea,we set different temperatures,pH values,culture media,and carbon and nitrogen sources,and the result showed its optimum temperature range was 23-28 ℃,the optimum pH value was 5,and the optimum culture medium was OMA.The growth of B.dothidea showed no obvious difference between the tested carbon and nitrogen sources.Besides,the pathogenicity was getting intense with the temperature rising and the lethal temperature was 55 ℃.The indoor antifungal test showed an inhibition rate of 20％propiconazole microe-mulsion was above 90％.
Botryosphaeria dothidealeaf spot diseasebiological character
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万方数据
