Application of Autonomous Embryonic Gene BBM1 in Apomixis of Rice
Fixation of the heterosis of F,generation is the strategic goal and development direction of hybrid rice breeding,and apomixis is the most possible way to fix heterosis.In this study,the autonomous embryonic gene BBM1 was driven by constitutive promoter Ubiq.Through flow cytometry ploidy detection and gene chip analysis,haploid plants were found in T1 strains.Subsequently,the BBM1 gene expression box was driven by the egg-specific promoter AtDD45 and constructed into the gene editing vectors of PAIR1,REC8 and OSD1(MiMe).Cloned seeds were obtained,and the induction rate was as high as 95.24％.This study provides a reference for the application of apomixis technology system in rice.
ricefixation of heterosisBBM1 geneapomixisclone seedinduction rate
万方数据
维普
