Construction of DNA Fingerprint Map of Rice Varieties and Rapid Identification of Variety Purity
To construct the fingerprint of rice varieties tested in the regional trials of Sichuan Province in 2021 and establish a method for rapid identification of hybrid rice seed purity,this study combined SSR molecular marker detection technology,variety fingerprint data,single seed DNA extraction and capillary electrophoresis system and completed the fingerprint construction and genetic diversity analysis of the rice varieties tested in the regional trials of Sichuan Province in 2021.The results of seed purity identification of hybrid rice Huiheyou 566 were obtained quickly.The results showed that fingerprinting of rice varieties,DNA extraction from single seeds and capillary electrophoresis could help breeders to identify seed purity faster.