培育转基因水稻干扰褐飞虱糖运输蛋白基因6的研究
Studies on Transgenic Rice to Interfere with Brown Planthopper Sugar Transporter Protein Gene 6
谢坤 1李娜娜 1丁汉凤 1白静 1丛韫喆 1李广贤 2姚方印 2杨永义1
作者信息
- 1. 山东省农业科学院农作物种质资源研究所,山东济南 250100
- 2. 山东省农业科学院湿地农业与生态研究所,山东济南 250100
- 折叠
摘要
褐飞虱是危害水稻的重要害虫,对水稻的生产造成严重破坏.选取对褐飞虱生长发育有重要影响的糖运输蛋白基因6(Nlst6)为靶标基因,构建表达靶标基因的dsRNA的RNAi载体,通过农杆菌侵染转化培育转基因水稻,筛选获得表达靶标基因的dsRNA和siRNA的转基因水稻材料.褐飞虱取食转基因水稻后,体内靶标基因的转录水平显著降低,雌虫生殖力及后代若虫体重均显著降低,但未有致死表型出现.综上,通过培育转基因水稻干扰褐飞虱的生长发育来防治褐飞虱成为可能,为后续转基因抗褐飞虱水稻的培育提供依据.
Abstract
Brown planthopper(BPH)is an important pest to rice,which has caused serious damage to rice production.The sugar transporter protein gene 6(Nlst6),which has an important effect on the growth and development of BPH,was selected as the target gene to construct the RNAi vector expressing dsRNA of the target gene,to screen transgenic rice materials cultivated through agrobacterium infection and transformation expressing dsRNA and siRNA of the target gene.After feeding on transgenic rice,the transcription level of the target gene in brown planthopper was significantly reduced,female fertility and the weight of offspring nymphs were significantly decreased,but no lethal phenotype appeared.In conclusion,it is possible to control BPH by breeding transgenic rice to interfere with the growth and development of BPH,which provides a basis for the subsequent cultivation of transgenic rice resistant to BPH.
关键词
褐飞虱/RNAi/糖运输蛋白基因6/转基因水稻Key words
brown planthopper/RNA interference/sugar transporter protein gene 6/transgenic rice引用本文复制引用
基金项目
山东省重点研发计划(重大科技创新工程)(2021LZGC025)
山东省农科院农业科技创新工程项目(CXGC2023C02)
出版年
2024
国家科技期刊平台
NSTL
万方数据