Analysis of Functional Genes in Long-grain Japonica CMS Lines,Restorer Line and Their Hybrid Combinations
The hybrid combinations derived from the long-grain japonica rice CMS lines Jiahe 212A and Jiahe 549A and the restorer line NP001 had the advantages of wide adaptability,high yield,excellent grain quality and good resistance.To analyze the characteristics of long-grain japonica CMS lines and restorer lines and the heterosis of their hybrid combinations,and to explore their excellent functional genes,the SLYmlR high-density rice whole genome SNP chip was used to detect and analyze the possibly related major functional genes in Jiahe 212A,Jiahe 549A,NP001,Zhongheyou 1(Jiahe 212A/NP001)and Zhongheyou 5(Jiahe 549A/NP001).The results showed that the proportion of japonica rice segments in the genome of the CMS lines Jiahe 212A and Jiahe 549A was more than 85.00％,indicating that they belong to the japonica type.The number of indica rice and japonica rice segments in the genome of the restorer line NP001 accounted for 66.96％and 19.58％,respectively,indicating that it belongs to the indicaclinous type.GS3 and GW7/GL7 genes regulated the long-grain traits of Jiahe 212A and Jiahe 549A,and the ALKc allele and Wxbhaplotype made the sterile lines and the restorer line and their hybrid combinations have higher gelatinization temperature and lower amylose content.The genes Pizt,Pid3,Pi-d2/Pid2,Pii/HIT7/pi5-1 and Pi-ta increased blast resistance of the sterile lines and the restorer line,while the genes Xa26/Xa3 increased the resistance of bacterial leaf blight.The wide compatibility genes SaF and S5-5/ORF5n were found in the sterile lines and the restorer line,respectively.In addition,Zhongheyou 1 and Zhongheyou 5 and their parents carried a large number of superior alleles,and functional gene complementarity was achieved between the sterile line and the restorer line in plant type construction,flowering time,increased grain yield and increased blast resistance.The results provided a scientific basis for further improvement of parental breeding and utilization of intersubspecific hybrid combinations between indica and japonica rice.
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