Comparative Study on the Methods for Extracting Total RNA from Mature Rice Seeds
In order to find out the optimal extraction method for obtaining high-quality total RNA from mature rice seeds,five extraction methods including PLANTeasy plant RNA extraction kit(PLANTeasy method),Trizol method,EASYspin plant RNA rapid extraction kit(EASYspin method),CTAB(hexadecyltrimethy ammonium bromide)-LiCl precipitation method and modified CTAB+column method were studied comparatively.The quality and purity of total RNA samples were detected by agarose gel electrophoresis,ultraviolet analysis and Agilent 2100 biological analyzer,and the target band of actin gene was detected by reverse transcription PCR(RT-PCR).The results showed that the quality and purity of total RNA samples extracted by the EASYspin method and the modified CTAB+column method were relatively higher than those by the other three methods.A bright and single band of the target gene was obtained by RT-PCR amplification using total RNA extracted by these two methods as template.Particularly,the modified CTAB+column method can obtain a high yield and good integrity of total RNA,so it is more suitable for the extraction of total RNA from rice seeds.
万方数据
维普
