Optimization of Flow Cytometry and Its Application in Identification of Apomictic Materials in Rice
Artificial creation of rice apomixis system is an effective way to fix heterosis.The offspring of rice apomixis contain diploid asexual seeds and tetraploid sexual seeds.Therefore,accurate identification of these two ploidy types in the offspring is crucial for apomixis research in rice.A comparative study was conducted using flow cytometry(FCM)to investigate the effects of different developmental stages and sampling parts of rice on ploidy detection results.The aim was to determine the appropriate developmental stage and optimal sampling part for ploidy identification of apomictic rice materials,and to verify the ploidy of their offspring through phenotypic observation and root tip chromosome preparation.The results showed that during the seedling stage,the relative DNA contents were fluctuated greatly among the same part of different rice plants and different parts of the same rice plant,whereas the relative DNA contents were stable in the leaf sheath.The relative DNA contents were most stable in the internodes of rice plants at tillering stage,which could be the optimal material for FCM detection of rice ploidy.These findings provide a scientific reference for the selection of materials for the identification of rice chromosome ploidy by FCM in apomixis research.
万方数据
维普
