摘要
利用稻瘟病抗性基因Pi2、Pi9、Pib、Pigm、Pikh、Pikm、Pita和Pi5的特异性分子标记,对104份早籼稻品系进行抗性基因鉴定与稻瘟病抗性评价.结果表明,在这些品系中,稻瘟病抗性基因Pigm的分布频率最高,达到66.35％,其次为 Pi5(10.58％)、Pib(2.88％)、Pikh(1.92％)、Pita(1.92％)、Pi2(0.96％);稻瘟病抗性等级达到"抗"的品系有78份,占比75.00％,其中携带抗病基因Pi2、Pikh和Pita的品系全部表现为抗病.包含2和3个抗性基因的品系分别有8和1份,其对应的抗病基因组合为Pi5+Pita、Pigm+Pita、Pigm+Pikh和Pi5+Pib+Pigm,这些品系在培育具有持久稻瘟病抗性的早籼稻品种上有较大的应用潜力.研究为抗病种质资源的挖掘和稻瘟病抗性基因在早籼稻育种中的应用提供参考.
Abstract
Using specific molecular markers of blast resistance genes Pi2,Pi9,Pib,Pigm,Pikh,Pikm,Pita and Pi5,the resistance genes and rice blast resistance of 104 early indica rice lines were identified and evaluated.The results showed that the distribution frequency of blast resistance gene Pigm was the highest,reaching 66.35％,followed by Pi5(10.58％),Pib(2.88％),Pikh(1.92％),Pita(1.92％)and Pi2(0.96％).There were 78 lines with"resistance"to rice blast,accounting for 75.00％,of which the lines carrying disease resistance genes Pi2,Pikh and Pita were all resistant.There were eight lines and one line containing two and three resistance genes,respectively.Their corresponding resistance gene combinations were Pi5+Pita,Pigm+Pita,Pigm+Pikh and Pi5+Pib+Pigm.These lines have great application potential in breeding of early indica rice varieties with durable blast resistance.This study provides a reference for the excavation of disease-resistant germplasm resources and the application of blast resistance genes in early indica rice breeding.
维普
万方数据