The expression analysis of drought tolerance-related genes in NO-related mutants under drought treatment conditions
About nitric oxide ( NO ) , a small gaseous molecule that regulated many biological processes in plants, was reported that Arabidopsis noa1 mutant, defective in NO production, was more resistant to water deficit than wilde-type Col-0 and the higher expression levels of a few drought-related genes were correlated with the enhanced drought tolerance of noa1.To confirm the results and further investigate the role of NO in drought tolerance, the plants of Col-0, noa1 as well as gsnor1-3, a GSNOR1 loss-of-function mutant, defec-tive in de-nitrosylation were subjected to drought treatment for various length of time and RT-PCR analysis was performed for total of 12 drought-related genes that belonged to 8 different gene families.The RT-PCR analysis indicated that no significant differences were observed for the expression of genes related to drought resistance/tolerance among Col-0, noa1 and gsnor1-3 and the drought tolerance displayed by noa1 might not result from higher expression of genes involved in drought tolerance.
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