为探究ICE(inducer of CBF expression)转录因子在高丛蓝莓花果发育进程中可能的调控机制,通过对VcICE基因家族进行全基因组鉴定及生物信息学分析,采用实时荧光定量PCR(qRT-PCR)技术分析VcICE基因亚家族成员在蓝莓花芽膨大和果实发育进程中的相对表达水平.结果表明:在高丛蓝莓'Draper'基因组中鉴定出 8 个VcICE成员,系统进化分析将其划分为 2 个亚家族,各亚家族成员均包含S-rich,bHLH和ACT等保守结构域;VcICE基因启动子区域富含光响应、植物激素、生长发育及非生物胁迫相关顺式作用元件;VcICE亚家族基因在高丛蓝莓'O'Neal'和'Bluerain'花芽膨大与果实发育进程中的相对表达差异显著.研究结果以期为深入了解VcICE转录因子在蓝莓花果发育进程中的功能提供理论参考.
Genome-wide identification and relative expression analysis of transcription factor VcICE gene family in highbush blueberry during flower and fruit development
ICEs(inducers of CBF expression)were usually known as typical MYC-type bHLH transcriptional factors regulating developmental processes in plants.However,the possible regulation mechanism of ICE dur-ing highbush blueberry flower bud and fruit development remained elusive.VcICE members were identified and analyzed based on the highbush blueberry genome 'Draper',and the expression levels of VcICE subfami-lies during 'O'Neal' and 'Bluerain' flower bud and fruit development were detected by quantitative real-time PCR.The results showed that VcICE family contained 8 candidate members,which could be grouped into 2 subfamilies with gene structures and conserved domains.VcICE promoters contained diverse cis-acting ele-ments in response to light,plant hormones,development and abiotic stress.The relative expression patterns of VcICE subfamilies were significantly different during 'O'Neal' and 'Bluerain' flower bud and fruit develop-ment.The presented results would be helpful for researchers to understand the possible functions of VcICE during highbush blueberry flower bud and fruit development.
blueberryICEgenome-wide identificationflower and fruit developmentexpression analysis
NETL
NSTL
万方数据
