Objective To explore the interaction of multi-target stool DNA(MT-sDNA),intestinal flora and environmen-tal factors in the development of colorectal cancer,so as to provide insights into pathogenesis study of colorectal can-cer.Methods A total of 54 cases of colorectal cancer from the First Affiliated Hospital of Ningbo University were in-cluded in the case group and 51 healthy subjects were included in the control group.Demographic information,diet and family history of colorectal cancer were collected by a questionnaire survey.MT-sDNA,intestinal flora,cancer anti-gen 19-9(CA19-9),carcinoembryonic antigen(CEA)and other tumor markers were detected.Interactions of MT-sDNA,intestinal flora and environmental factors with the development of colorectal cancer was analyzed by multifactor dimen-sionality reduction(MDR),crossover analysis and additive model.Results The case group included 20 males(37.04％)and 34 females(62.96％),and had a mean age of(64.89±9.72)years.The control group included 24 males(47.06％)and 27 females(52.94％),and had a mean age of(53.94±10.33)years.MDR analysis showed that subjects with both high absolute intestinal flora indexes and positive MT-sDNA had an increased risk of colorectal cancer(OR=3.782,95％CI:1.190-5.034).Crossover analysis showed that subjects with positive MT-sDNA and>5 μg/L of CEA had an in-creased risk of colorectal cancer(OR=2.121,95％CI:1.162-4.033).Additive model analysis showed that MT-sDNA had positive additive interaction with CEA(SI=3.687,95％CI:1.229-7.238),and MT-sDNA had negative additive interaction with fruit intake(SI=0.145,95％CI:0.020-0.753).Conclusion Positive MT-sDNA can synergistically increase the risk of colorectal cancer with high intestinal flora index and CEA,and fruit intake can reduce the risk of colorectal cancer in MT-sDNA-positive population.
维普
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