Objective To explore the effects of protein powder on the bioavailability of perfluoroalkyl substances(PFASs)in blood and kidneys of rats and renal function change.Methods Twenty-four rats of the SD strain were ran-domly divided into the negative control group,PFASs group and protein powder group,with 8 rats(half males and half females)in each group.PFASs included 13 perfluorocarboxylic acids(PFCAs)and 8 perfluorosulfonic acids(PFSAs),and the mixture was used as a test subject for intervention.The rats in the negative control group were given deionized water at doses of 20 mL/kg·bw,in the PFASs group were given 5 mL/kg·bw of PFASs mixtures and 15 mL/kg·bw of deionized water,and in the protein powder group were given 5 mL/kg·bw of PFASs mixtures and 15 mL/kg·bw of protein powder(0.258 g/mL).After intervention for 28 successive days,body weight and kidney mass were weighed,and the kidney volume index was calculated.Serum creatinine and blood urea nitrogen were detected by an automatic bio-chemical analyzer.The PFCAs,PFSAs and PFASs contents were quantified in blood and kidney using ultra-high perfor-mance liquid chromatography-electrospray tandem mass spectrometry,and the bioavailability was estimated.Results There was no significant differences in kidney mass,kidney volume index,serum creatinine and blood urea nitrogen among the negative control group,PFASs group and protein powder group(all P>0.05).The bioavailability of blood PF-CAs,PFSAs and PFASs in the protein powder group was not significantly different from the PFASs group(all P>0.05).Compared with the PFASs group,the bioavailability of PFCAs,PFSAs and PFASs were significantly increased in kid-neys of male rats in the protein powder group(all P<0.05),while were not significant different in those of female rats(all P>0.05).Conclusion Protein powder at the dose of this study can significantly improve the bioavailability of PFASs in kidneys of male rats,while there no obvious effects on the bioavailability of blood PFASs and renal function.
perfluoroalkyl substancesbioavailabilityprotein powderrenal function
万方数据
维普
