Objective To investigate the effects of decabromodiphenyl ether(BDE-209)on the size of subcutaneous transplanted tumors,related genes and signaling pathways of cervical cancer in mice.Methods Forty female C57BL/6 mice were subcutaneously inoculated with mouse cervical carcinoma U14 cells in the lateral axilla to establish a mouse subcutaneous transplanted tumor model.These mice were randomly divided into a high-dose group(500 mg/kg),a medi-um-dose group(100 mg/kg),a low-dose group(20 mg/kg)and a control group(corn oil),and were exposed to BDE-209 or corn oil by gavage.Subcutaneous transplanted tumor tissue was taken after 21 days of BDE-209 poisoning,and the differentially expressed genes in the subcutaneous transplanted tumors of cervical cancer among the four groups were analyzed by high-throughput transcriptome sequencing and were subjected to Gene Ontology(GO)annotations and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis.Protein-protein interactions(PPI)were analyzed using the STRING database,and the mRNA expression of hub genes was determined by real-time fluorescence poly-merase chain reaction.Results Compared with the control group,low-dose group and medium-dose group,the mass of subcutaneous transplanted tumors in the high-dose group was decreased(all P<0.05).Transcriptome sequencing results showed that compared with the control group,2 011 genes were up-regulated and 1 165 genes were down-regulated in the high-dose group;960 genes were up-regulated and 357 genes were down-regulated in the medium-dose group;537 genes were up-regulated and 262 genes were down-regulated in the low-dose group(all P<0.05).GO and KEGG analy-sis showed that the differentially expressed genes in the high-dose group were mainly involved in cell chemotaxis and NOD-like receptor signaling pathway;the differentially expressed genes in the medium-dose group were mainly involved in cell chemotaxis and cytokine-cytokine receptor interactions;and the differentially expressed genes in the medium-dose group were mainly involved in processing and presentation of antigens,and the signaling pathways of the comple-ment and coagulation cascades.Compared with the control group,the mRNA expression of TLR2,MMP9,IL-6,Fos,and TNF was up-regulated in the high-dose group(all P<0.05).Conclusion High-dose BDE-209 may affect Toll-like receptors,NOD-like receptors,and other immune and inflammatory-related signaling pathways and cancer-related genes,leading to a decrease in the mass of subcutaneous transplanted cervical cancer tumors in mice.
维普
万方数据