Objective To investigate protective effects of nicotinamide mononucleotide(NMN)on ethanol-induced DNA damage in L02 cells,so as to provide the evidence for adjuvant therapy of NMN on alcoholic liver diseases.Methods L02 cells were pretreated with different concentrations of NMN(0,1,2,4 and 8 mmol/L)for 6 h,and then were ex-posed to 0.4%ethanol for 12 h.The treated cells were divided into the control group,0.4%ethanol group and differ-ent concentrations of NMN groups.Cell viability was analyzed using trypan blue staining for determining the concentra-tion of NMN as a protective agent.The effects of NMN on ethanol-induced DNA damage in L02 cells were evaluated using immunofluorescence detection and reactive oxygen species(ROS)assay.L02 cells were exposed to 0.4%ethanol for 12 h,cultured in a medium containing a protective concentration of NMN,and divided into PBS group and NMN group.Cell viability was detected at 0,2,4,8,16 and 32 h,and the effects of NMN on repairing ethanol-induced DNA damage were evaluated by alkaline comet assay.Results The cell viability was lower in 0.4%ethanol group than than in the control group,and was higher in different concentrations of NMN groups than in 0.4%ethanol group(all P<0.05),with no significant difference in the cells viability between 4 mmol/L and higher concentrations of NMN groups and the control group(all P>0.05).Therefore,4 mmol/L NMN was selected as a protective agent.The cell tail moments,relative immunofluorescence intensities of γH2AX and relative levels of ROS were higher in 0.4%ethanol group than in the control group,and lower in 4 mmol/L and higher concentrations of NMN groups than in 0.4%etha-nol group(all P<0.05).The cell viability was increased and the cell tail moment was shortened with the increase of 4 mmol/L NMN intervention time;and the cell viability in 4 h and more of NMN groups were higher,and the cell tail moment were lower than that in PBS group(all P<0.05).Conclusions NMN attenuates DNA damage in a dose-depen-dent manner and promotes the repair of DNA damage in a time-dependent manner.NMN has a protective effect on eth-anol-induced DNA damage in hepatocytes.
关键词
烟酰胺单核苷酸/乙醇/DNA损伤/活性氧
Key words
nicotinamide mononucleotide/ethanol/DNA damage/reactive oxygen species
维普
万方数据