绍兴市MSM人群HIV-1分子传播网络特征分析
HIV-1 molecular transmission network among men who have sex with men in Shaoxing City
何婷婷 1曹栋卿 1林家锋 1蒋卓婧 1张佳峰 2范钦2
作者信息
- 1. 绍兴市疾病预防控制中心微生物检验科,浙江 绍兴 312071
- 2. 浙江省疾病预防控制中心,浙江 杭州 310051
- 折叠
摘要
目的 分析浙江省绍兴市男男性行为人群(MSM)艾滋病病毒1型(HIV-1)分子传播网络特征,为MSM人群艾滋病防治提供依据.方法 选择2021年1月—2023年9月绍兴市新报告MSM人群艾滋病病毒感染者和艾滋病患者(HIV/AIDS)为研究对象,收集抗病毒治疗前血浆样本,采用反转录PCR和巢式PCR扩增HIV-1 pol基因,构建系统进化树分析基因亚型;采用HIV-TRACE法构建1.5%基因距离的分子传播网络,分析成簇情况和分子簇内病例特征.结果 纳入2021年1月—2023年9月绍兴市MSM人群HIV/AIDS病例216例,获得合格序列179例.HIV-1亚型以CRF07_BC和CRF01_AE为主,分别为95和66例.在1.5%基因距离下共形成20个分子簇,61个节点和58条边.61条序列入网,入网率为34.08%.绍兴市各县(市、区)均有MSM人群HIV/AIDS病例入网.节点数≥10个的大分子簇1个,涉及5个县(市、区)12例病例;节点数4～5个的中分子簇3个;节点数2～3个的小分子簇16个.边数≥4条的高传播风险病例有7例,均为CRF07_BC亚型;其中2例来自大分子簇,5例来自嵊州市、新昌县病例组成的同一分子簇.结论 绍兴市MSM人群HIV/AIDS病例HIV-1基因亚型以CRF07_BC和CRF01_AE为主,存在跨地区HIV传播,嵊州市和新昌县可能存在潜在传播风险点.
Abstract
Objective To investigate the molecular transmission network characteristics of HIV-1 among men who have sex with men(MSM)in Shaoxing City,Zhejiang Province,so as to provide insights into AIDS prevention and control among MSM.Methods Newly reported HIV/AIDS cases among MSM in Shaoxing City were selected from January 2021 to September 2023.Plasma samples before the antiviral treatment were collected.The HIV-1 pol gene was ampli-fied using reverse transcription PCR and nested PCR to construct phylogenetic trees for gene subtype analysis.The HIV-TRACE method was used to construct a molecular transmission network with a genetic distance of 1.5%to ana-lyze clustering and the characteristics of cases within molecular clusters.Results A total of 216 HIV/AIDS cases among MSM were included,and 179 qualified sequences were obtained.The predominant HIV-1 subtypes were CRF07_BC and CRF01_AE,with 95 and 66 cases,respectively.At 1.5%genetic distance,20 molecular clusters were formed,with 61 nodes and 58 edges.A total of 61 sequences were connected to the transmission network(34.08%).HIV/AIDS cases among MSM from all the counties(cities,districts)in Shaoxing City were included in the network.There was the largest molecular cluster with≥10 nodes,involving 12 cases from five counties(cities,districts),3 medi-um-sized molecular clusters with 4 to 5 nodes,and the 16 small-sized molecular clusters with 2 or 3 nodes.Seven cas-es with high risk of transmission,each with≥4 edges,were all CRF07_BC subtypes.Among them,two cases were from the large molecular cluster,and five cases were from the same molecular cluster composed of cases from Sheng-zhou City and Xinchang County.Conclusions The predominant HIV-1 subtypes among MSM in Shaoxing City were CRF07_BC and CRF01_AE.There was cross-regional HIV transmission,and potential transmission risk might exist in Shengzhou City and Xinchang County.
关键词
艾滋病/男男性行为人群/分子传播网络/基因亚型Key words
AIDS/men who have sex with men/molecular transmission network/gene subtype引用本文复制引用
基金项目
绍兴市科技计划项目(2018C30051)
绍兴市科技计划项目(2022A14007)
出版年
2024
NETL
NSTL
万方数据