Objective To investigate the molecular transmission network characteristics of HIV-1 among men who have sex with men(MSM)in Shaoxing City,Zhejiang Province,so as to provide insights into AIDS prevention and control among MSM.Methods Newly reported HIV/AIDS cases among MSM in Shaoxing City were selected from January 2021 to September 2023.Plasma samples before the antiviral treatment were collected.The HIV-1 pol gene was ampli-fied using reverse transcription PCR and nested PCR to construct phylogenetic trees for gene subtype analysis.The HIV-TRACE method was used to construct a molecular transmission network with a genetic distance of 1.5%to ana-lyze clustering and the characteristics of cases within molecular clusters.Results A total of 216 HIV/AIDS cases among MSM were included,and 179 qualified sequences were obtained.The predominant HIV-1 subtypes were CRF07_BC and CRF01_AE,with 95 and 66 cases,respectively.At 1.5%genetic distance,20 molecular clusters were formed,with 61 nodes and 58 edges.A total of 61 sequences were connected to the transmission network(34.08%).HIV/AIDS cases among MSM from all the counties(cities,districts)in Shaoxing City were included in the network.There was the largest molecular cluster with≥10 nodes,involving 12 cases from five counties(cities,districts),3 medi-um-sized molecular clusters with 4 to 5 nodes,and the 16 small-sized molecular clusters with 2 or 3 nodes.Seven cas-es with high risk of transmission,each with≥4 edges,were all CRF07_BC subtypes.Among them,two cases were from the large molecular cluster,and five cases were from the same molecular cluster composed of cases from Sheng-zhou City and Xinchang County.Conclusions The predominant HIV-1 subtypes among MSM in Shaoxing City were CRF07_BC and CRF01_AE.There was cross-regional HIV transmission,and potential transmission risk might exist in Shengzhou City and Xinchang County.
AIDSmen who have sex with menmolecular transmission networkgene subtype
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