舟山市HIV/AIDS病例HIV-1分子传播网络分析
HIV-1 molecular transmission network among HIV/AIDS cases in Zhoushan City
陈圆静 1李科峰 1范钦 2张佳峰 2吴铭宇 1张波 3顾松叶 1王海 1程伟2
作者信息
- 1. 舟山市疾病预防控制中心艾滋病结核病防制科,浙江 舟山 316021
- 2. 浙江省疾病预防控制中心,浙江 杭州 310051
- 3. 舟山市定海区疾病预防控制中心,浙江 舟山 316000
- 折叠
摘要
目的 了解浙江省舟山市艾滋病病毒感染者和艾滋病患者(HIV/AIDS)艾滋病病毒1型(HIV-1)分子传播网络特征.方法 选择2020-2022年舟山市新报告的HIV/AIDS病例为研究对象,首次随访时收集基本信息并采集全血标本,采用反转录PCR和巢式PCR扩增HIV-1 pol区序列,构建Neighbor-Joining系统进化树鉴定HIV-1亚型,采用Cytoscape 3.6.1软件构建HIV-1分子传播网络进行分析.结果 2020-2022年舟山市新报告HIV/AIDS病例222例,收集全血样本200例,检测获得合格序列152例,其中男性122例,占80.26%;年龄≥50岁75例,占49.34%;初中及以下文化程度109例,占71.71%;经商业异性性行为传播63例,占41.45%.检出HIV-1亚型以CRF07_BC和CRF01_AE为主,分别占45.39%和21.05%.基因距离阈值设为1%时,有69例HIV/AIDS病例形成20个分子簇,成簇率为45.39%;以2020年报告的HIV/AIDS病例构成的分子网络为对照,2022年新增病例数≥5例的活跃分子簇有2个,各9例病例,以年龄≥50岁、初中及以下文化程度和经商业异性性性行为传播为主要特征.结论 舟山市HIV/AIDS病例HIV-1优势型为CRF07_BC和CRF01_AE,中老年人商业异性性行为传播是主要传播模式.
Abstract
Objective To investigate the characteristics of HIV-1 molecular transmission network among HIV/AIDS pa-tients in Zhoushan City,Zhejing Province.Methods The newly reported HIV/AIDS cases in Zhoushan City from 2020 to 2022 were selected.Basic information was collected and whole blood samples were obtained at the initial follow-up.The pol gene sequences of HIV-1 were amplified by RT-PCR and nested-PCR.HIV-1 subtypes were identified by Neighbor-Joining phylogenetic trees.The HIV-1 molecular transmission network was built and analyzed using Cytoscape 3.6.1 software.Results A total of 222 HIV/AIDS cases were reported in Zhoushan City from 2020 to 2022,200 whole blood samples were collected,and 152 sequences were obtained successfully,including 122 males(80.26%),75 cases aged 50 years and above(49.34%),109 cases with a junior high school education or below(71.71%),and 63 cases with commercial heterosexual contact(41.45%).The main subtypes were CRF07_BC and CRF01_AE,accounting for 45.39%and 21.05%,respectively.When the threshold of genetic distance was set to 1%,20 molecular clusters were formed in 69 cases,with a clustering rate of 45.39%.Using the molecular network constituted by reported HIV/AIDS cases in 2020 as the baseline network,there were 2 active molecular clusters with≥5 new cases in 2022,each with 9 cases,characterized mainly by individuals aged 50 or above,with a junior high school education or below,and transmis-sion through commercial heterosexual sex.Conclusion The predominant HIV-1 subtypes among HIV/AIDS cases in Zhoushan City are CRF07_BC and CRF01_AE.Transmission through commercial heterosexual contact among middle-aged and elderly people is a main mode of HIV transmission.
关键词
艾滋病病毒/分子传播网络/分子簇Key words
HIV/molecular communication network/molecular cluster引用本文复制引用
基金项目
浙江省科技计划项目(2024C35088)
舟山市医药卫生科技计划项目(2023YA12)
出版年
2024
NETL
NSTL
万方数据