Effect evaluation of using normal saline to wash red blood cells for blood type identification in patients with disseminated intravascular coagulation
Objective To evaluate the effectiveness of blood type identification after red blood cell washing in patients diagnosed with disseminated intravascular coagulation(DIC)caused by self-aggregating red blood cells.Methods A total of 24 samples from DIC patients with self-aggregation red blood cell from July 2019 to July 2022 in Hangzhou Third People's Hospital in Zhejiang Province were collected.ABO blood type was based on genotyping results.The results of ABO blood type identification by tube method and micro-column gel agglutination assay(MGAA)before and after washing aggregated red blood cells with normal saline were compared with those of the standard group.Results The positive and negative ABO blood types of 2 cases were coincident before red blood cell washing,and there was significant difference between the results and the standard results(P<0.05).The red blood cells dispersed evenly after washing with normal saline.The results of ABO blood type were positive and negative in 24 cases,including 8 cases of type A,5 cases of type B,9 cases of type O and 2 cases of type AB.There was no significant difference between the standard results and the blood type results after washing(P>0.05).Conclusions The method of washing red blood cells with normal saline has a good blood type identification effect,which has obvious advantages in detection,and provides a safety guarantee for clinical emergency blood use.
Self-aggregating red blood cellsRed blood cell washingBlood typeDisseminated intravascular coagulation
万方数据
维普
