Effect of Periplaneta Americana powder on oxidative stress in rats with spinal cord injury
Objective To investigate the effect of Periplaneta Americana powder on oxidative stress in the spinal cord injury area of rats with hemi-transected spinal cord injury(hSCI).Methods Sixty female SD rats were randomly divided into a control group and an observation group,with 30 rats in each group.The hSCI model was established in both groups by opening the T10 vertebral plate to perform left spinal cord hemisection.Thirty min-utes before surgery and 1～14 days after surgery,the control group was administered 0.9%sodium chloride solution,while the observation group was given Periplaneta Americana powder(630 mg/kg)dissolved in 0.9%sodium chloride solution via gavage,5 mL per rat,once daily.Five rats were taken from each group at each time point 6 hours and 1,3,7,and 14 days after surgery,and the malondialdehyde(MDA)and 8-hydroxydeoxyguanosine(8-OHdG)contents were detected by enzyme-linked immunosorbent assay.Five rats were taken from each group 14 d after surgery for Nissl staining of spinal cord tissue.Results Compared with the control group,the observation group showed a significantly greater number of neurons in the spinal cord tissue under the microscope field of view of 10×20 and 10×40[(26.25±0.50)vs.(10.84±0.58),(14.92±0.32)vs.(6.75±0.32),P<0.01].The observation group had lower levels of MDA[(0.61±0.03)nmol/mL vs.(0.89±0.02)nmol/mL,(0.54±0.01)nmol/mL vs.(0.68±0.02)nmol/mL,P<0.01]and 8-OHdG[(17.92±0.99)ng/mL vs.(23.37±0.77)ng/mL,(15.91±0.28)ng/mL vs.(20.18±0.37)ng/mL,P<0.01]in the spinal cord tissues at days 7 and 14 after surgery.Compared with the control group,the peak time of ox-idative stress in the observation group occurred earlier,and the peak values of MDA and 8-OHdG expression were advanced to 3 days after surgery.Conclusion Periplaneta Americana powder reduced the expression of MDA and 8-OHdG in the spinal cord injury area of hSCI rats,advanced the peak time of oxidative stress,alleviated oxida-tive stress damage,and reduced neuronal loss.
RatSpinal cord injuryPeriplaneta Americana powderOxidative stressMalondialdehyde8-hy-droxy-2'-deoxyguanosine
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