miR-378a-3p靶向NUAK2抑制乳腺癌细胞的增殖、迁移和侵袭
miR-378a-3p Targets NUAK2 to Inhibit Proliferation,Migration,and Invasion of Breast Cancer Cells
陈建彬 1王泰人2
作者信息
- 1. 318000 台州,台州市立医院肿瘤外科
- 2. 310000 杭州,杭州熙禾医院整形外科
- 折叠
摘要
目的 探讨微小RNA-378a-3p(miR-378a-3p)影响乳腺癌细胞发展的机制.方法 基于TCGA数据库分析miR-378a-3p在乳腺癌细胞中的表达情况;利用starBase、miRDB、miRWalk数据库对miR-378a-3p进行靶基因预测,双荧光素酶报告实验验证miR-378a-3p对NUAK家族激酶2(NUAK2)的靶向调控;qRT-PCR和Western blot检测miR-378a-3p和NUAK2在乳腺癌细胞中mRNA和蛋白的表达情况;细胞增殖实验检测乳腺癌细胞的增殖能力;细胞划痕实验和侵袭实验分别检测乳腺癌细胞的迁移和侵袭能力.凋亡和周期实验检测乳腺癌细胞的凋亡率和细胞周期分布.结果 miR-378a-3p在乳腺癌细胞中显著下调,过表达miR-378a-3p抑制了乳腺癌细胞的增殖,迁移和侵袭.miR-378a-3p靶向下调NUAK2的表达.结论 miR-378a-3p通过靶向抑制NUAK2的表达,从而抑制乳腺癌细胞的增殖、迁移和侵袭.
Abstract
Objective To explore the mechanism of microRNA-378a-3p(miR-378a-3p)affecting the deve-lopment of breast cancer(BC)cells.Methods The expression of miR-378a-3p in BC cells based on the Cancer Genome Atlas Program database was analyzed.The starBase,miRDB,and miRWalk databases were used to predict the target genes of miR-378a-3p.Double-luciferase reporter experiments were performed to verify the targeted regulation of miR-378a-3p on NUAK family kinase 2(NUAK2).Real-time fluorescence quantitative PCR and Western blot analyses were used to detect the expression of miR-378a-3p and NUAK2 mRNA and protein in BC cells.Cell proliferation ability was detected through cell-proliferation experiments.Cell scratch assay and invasion assay were used to detect the migration and invasion abilities of cells,respectively.Apoptosis and cell-cycle experiments were conducted to detect cell apoptosis rate and cell cycle distribution.Results The expression of miR-378a-3p was significantly downregulated in BC cells,and miR-378a-3p overexpression inhibited the proliferation,migration,and invasion of BC cells.miR-378a-3p directly targeted NUAK2 and inhibited the mRNA and protein expression of NUAK2.Conclusion miR-378a-3p inhibits the proliferation,migration,and invasion of BC cells by targeting NUAK2.
关键词
乳腺癌/miR-378a-3p/NUAK2/增殖/迁移/侵袭Key words
Breast cancer/miR-378a-3p/NUAK2/Proliferation/Migration/Invasion引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据