Synthesis and characterization of lactoferrin modified chitosan magnetic nanoparticles and its uptake by HT-22 hippocampal cells
Objective To synthesize and characterize Fe3O4 loaded lactoferrin modified amphiphilic chitosan (Lf-QMC) magnetic nanoparticles (NPs),and to determine their affinity to HT-22 hippocampal cells and the in vivo brain targeting induced by magnetic field.Methods Oleic acid (OA) modified Fe3O4 (OA-Fe3O4) were loaded into Lf-QMC NPs by solvent evaporation method to obtain OA-Fe3O4-Lf-QMC NPs.The drug loading content of OA-Fe3O4-Lf-QMC NPs was 1.5％.The characterization of OA-Fe4O4-Lf-QMC was measured by FITR,TEM,DLS,XRD and VSM.Resazurin was used to ascertain the cytotoxicity of OA-Fe3O4-Lf-QMC Nps on HT-22 hippocampal cells.The uptake of OA-Fe3O4-Lf-QMC NPs by HT-22 hippocampal cells was investigated by TEM.Fluorescence analysis was used to examine the brain targeting of OA-Fe3O4-Lf-QMC NPs in vivo.Results OA-Fe3O4-Lf-QMC NPs were developed successfully with the saturation magnetic induction intensity of 1.2 emu · g-1.These magnetic NPs had no distinct cytotoxicity on HT-22 hippocampal cells at 2-200 μg · mL-1.In addition,they had good affinity with HT-22 hippocampal cells and could efficiently enter these cells.Under the guidance of magnetic field,OA-Fe3O4-Lf-QMC NPs exhibited more desirable brain targeting than Lf-QMC NPs in vivo.Conclusion OA-Fe3O4-Lf-QMC NPs are expected to deliver drug to nerve cells through blood-brain barrier due to the double targeting effect of magnetic field and lactoferrin.
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