Objective To investigate the chemical compositions from rhizomes of Chloranthus multistachys Pei..Methods The compounds were isolated by column chromatography with silica gel,Sephadex LH-20 and preparative HPLC,and the structures were elucidated by the physical and chemical methods and spectral data.Results Nine compounds were isolated,and their structures were determined as securigran Ⅰ (1),4α,8β-dihydroxy-5α (H)-eudesm-7 (11)-en-8,12-olide (2),chloraeudolide (3),zedoarolide B (4),serralactone C (5),peroxiatractylenolide Ⅲ (6),spicachlorantin B (7),shizukaol C (8),and 3-(3,4-dihydroxyphenyl)-acrylicacid 1-(3,4-dihydroxyphenyl)-2-methoxycarbonylethyl ester (9).Conclusion Compound 1-9 have been isolated from this plant for the first time.