高效液相色谱法同时测定干姜甘草配伍后6-姜辣素、甘草酸和甘草苷的含量变化
HPLC determination of 6-gingerol, glycyrrhizic acid and liquiritin contents after Zingiberis Rhizoma compatibility with Glycyrrhizae Radix et Rhizoma
李洁 1任爱农 1赵妍1
作者信息
- 1. 南京中医药大学附属中西医结合医院,南京210046;江苏省中医药研究院,南京210028
- 折叠
摘要
目的 比较干姜与甘草合煎后6-姜辣素、甘草酸和甘草苷含量的变化.方法 采用HPLC法同时测定干姜、甘草单煎液以及不同配伍比例合煎液中6-姜辣素、甘草酸和甘草苷含量的变化.结果 与单煎液比较,干姜与甘草合煎液中6-姜辣素、甘草酸含量均有所下降,其中干姜与甘草1∶2、1∶3的合煎液中6-姜辣素的含量分别降低7.50％、15.63％,甘草酸的含量分别降低19.16％、24.48％.结论 干姜与甘草合煎后,两者代表性有效成分6-姜辣素和甘草酸含量根据药材配伍比例不同,有不同程度的降低,甘草苷的含量变化无明显差异.初步揭示了甘草缓和干姜峻烈之性的物质基础所在,但其深层次的作用机制有待于进一步研究.
Abstract
Objective To compare the contents of 6-gingerol,glycyrrhizic acid and liquiritin in Zingiberis Rhizoma-Glycyrrhizae Radix et Rhizoma (ZR-GRR) mixed decoction.Methods Single GRR decoction,ZR decoction and different compatibility proportion ZR-GRR mixed decoction were taken as test samples,the content change of 6-gingerol,glycyrrhizic acid and liquiritin was measured simultaneously by HPLC.Results Compared with GRR decoction and ZR decoction,the contents of 6-gingerol and glycyrrhizic acid were both reduced;the contents of 6-gingerol and glycyrrhizic acid decreased by 7.50％ and 15.63％;and 19.16％ and 24.48％ in ZR-GRR mixed decoction at a ratio of 1 ∶ 2 and 1 ∶ 3,respectively.Conclusion The effective contents of components (6-gingerol and glycyrrhizic acid) in ZR-GRR mixed decoction decrease to some extent in different compatibility proportions of ZR-GRR mixed decoction.The liquiritin variation is not obviously different,indicating the material basis that GRR can relieve the strong nature of ZR,but the concrete mechanism need further study.
关键词
干姜甘草合煎液/高效液相色谱法/6-姜辣素/甘草酸/甘草苷/含量变化Key words
Zingiberis Rhizoma-Glycyrrhizae Radix et Rhizoma mixed decoction/HPLC/6-gingerol/glycyrrhizic acid/liquiritin/content change引用本文复制引用
基金项目
江苏省基础建设项目(BM2008152/Y1208)
出版年
2016
NETL
NSTL
维普
万方数据