Flavonoids from Sichuan Aurantii Fructus and their effects on the proliferation of osteoblasts
Objective To determine the chemical constituents of flavonoids from Sichuan Aurantii Fructus and their effects on the proliferation of osteoblasts.Methods Polyamide resin,dextran gel,silica gel column chromatography and other methods were used to separate the components.The structures were analyzed and identified by mass spectrometry and nuclear magnetic resonance spectroscopy.The obtained compounds were tested for osteoblast proliferation activity by in vitro cell experiments.Results Twelve flavonoids and their glycosides were isolated from Sichuan Aurantii Fructus,and were identified as naringin(1),neohesperidin(2),naringenin-7-O-β-D-glucopyranoside(3),narirutin(4),sarotanoside(5),eriodictyol-7-O-β-D-glucopyranoside(6),naringenin(7),genkwanin(8),eriodictyol(9),angophorol(10),choerospondin(11),and tetrahydroprotogenkwanone(12).Compounds 1～4,6～9 and 12 promoted osteoblast proliferation,and the cell proliferation rate of compound 12 reached 196.3％,which was superior to that of estradiol.Conclusion Compounds 5,8,10,11,and 12 have been isolated from Aurantii Fructus for the first time,while 5,10,and 12 from Citrus for the first time.Compounds 1～4,6～9 and 12 obviously promote the proliferation of osteoblasts.
万方数据
维普
