Changes in inflammatory factors in a model of lipopolysaccharide-induced airway epithelial cell injury
Objective To determine the regulatory effect of lipopolysaccharide(LPS)on the secretion of inflammatory cytokines after epithelial cell(BEAS-2B)injury in the airway.Methods LPS was used to induce inflammatory injury in BEAS-2B.Cell supernatants were collected at 3 h,6 h,12 h and 24 h after the stimulation,and the levels of IL-8,IL-6,IL-17,ILL-1β,TSLP,IL-13,IL-33 and MCP-1 in the cell supernatants were measured by ELISA,and NO levels in cell supernatants were detected via nitrate reduction.Results LPS promoted the secretion of IL-8,IL-6,IL-17 and MCP-1 in BEAS-2B cells,and all the induction time(3,6,12,and 24 h)greatly increased the levels of IL-8,IL-6,IL-17 and MCP-1 factors,but the peak secretion time of different factors was not consistent.In addition,LPS did not significantly stimulate the secretion of IL-1β,TSLP,IL-13,IL-33 and NO in BEAS-2B cells.Conclusion The LPS-stimulated BEAS-2B cell injury model is suitable in the mechanism study on the regulation of IL-8,IL-6,IL-17 and MCP-1 factors.
万方数据
维普
