Preparation and in vitro release of resveratrol nanostructured lipid carriers
Objective To prepare resveratrol nanostructured lipid carriers(Res-NLCs)and comprehensively assess their physical and chemical attributes,as well as in vitro release characteristics.Methods Res-NLCs were formulated with the emulsification evaporation-high-pressure homogenization method.An orthogonal experimental design was used to optimize the formulation.The physical and chemical properties of Res-NLCs were meticulously analyzed.An HPLC method was established to quantify the in vitro resveratrol release,while forward dynamic membrane dialysis was used to evaluate drug release kinetics.Results The optimal Res-NLC formulation consisted of 0.0356 g glycerol stearate,0.3918 g soybean phospholipid,a 4.25％mass concentration of Tween-80,and 0.0475 g polyethylene glycol caproate.Transmission electron microscopy revealed that Res-NLCs were spherical and well-dispersed.Res-NLC particles had an average size of(81.87±0.14)nm,a polydispersity coefficient of(0.099±0.02),and a Zeta potential of(-8.09±0.75)mV.These Res-NLCs exhibited high encapsulation efficiency(98.43±0.07)％and drug loading(11.04±0.14)％.In vitro release studies indicated that the resveratrol solution released 97.31％at 8 hours,while Res-NLCs released sustainably.Subsequently,Res-NLCs reached a release plateau after 48 h,with a cumulative release of 80.69％.Conclusion Res-NLCs prepared via emulsification evaporation-high-pressure homogenization,exhibit small and uniformly distributed particles,high encapsulation efficiency,and substantial drug loading capacity.Extension of the release duration of resveratrol,demonstrate an effective sustained release profile.
万方数据
维普
