Correlation and value transfer of HPLC characteristic chromatogram of raw herbs,decoction pieces,standard decoction and formula granules of Flos Sophora Immaturus
Objective To establish the HPLC characteristic chromatogram of raw herbs,decoction pieces,standard decoction,and formula granules of Flos Sophora Immaturus and determine their correlation and quantity transfer.Methods Agilent ZORBAX SB-C18 column(4.6 mm×250 mm,5 μm)was used with the mobile phase consisting of acetonitrile and 0.1％phosphoric acid aqueous with gradient elution.The flow rate was 1.0 mL·min-1,the detection wavelength was 257 nm and the column temperature was 30 ℃.In contrast with the reference characteristic chromatogram,the characteristic chromatogram and correlation of 15 batches of Flos Sophora Immaturus raw herbs-decoction pieces-standard decoction-formula granules were compared,and the quantity value transfer laws were analyzed.Results Totally 7 common peaks were confirmed in the HPLC characteristic chromatogram of 15 batches of raw herbs,decoction pieces,standard decoction,and formula granules of Flos Sophora Immaturus showed 7 characteristicn narcissoside,quercetin,isorhamnetin could be identified.The similarity of characteristic chromatogram of raw herbs,decoction pieces,standard decoction,and formula granules of Flos Sophora Immaturus was all greater than 0.90.The main chemical compositions were basically the same,with good correlation.The content and transfer rate of index components were determined and no dispersion was found,suggesting good transferability.Conclusion The HPLC characteristic chromatogram of raw herbs,decoction pieces,standard decoction,formula granules of Flos Sophora Immaturus is established,reflecting the overall appearance of multiple components.
Flos Sophora Immaturus raw herbdecoction piecestandard decoctionformula granuleHPLCcharacteristic chromatogramcorrelation
万方数据
维普
