Objective To improve the quality standard of Sarcandra glabra.Methods Thirteen batches of Sarcandra glabra from different areas were used as the research object.As a control substance,isofraxidin and standard herb were identified by thin layer chromatography(TLC).The moisture,total ash,acid-insoluble ash,and extract of Sarcandra glabra were determined according to the general principles in Chinese Pharmacopoeia(2020 Edition).The content of isofraxidin and rosmarinic acid was determined by high-performance liquid chromatography(HPLC).Results The spots of TLC were clear and well separated.The content of moisture,total ash,acid-insoluble ash and extract of samples was 9.4%,5.2%,0.5%and 12.6%,respectively.The content of isofraxidin and rosmarinic acid was 0.026%and 0.030%,respectively.Conclusion This method is highly specific,accurate and simple,which may be used in the quality control of Sarcandra glabra.