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九节茶药材质量标准研究

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目的 对九节茶药材开展全面的质量标准研究,建立完善的九节茶质量标准.方法 收集13批九节茶药材,以异嗪皮啶对照品和九节茶对照药材建立薄层色谱鉴别方法;参考《中国药典》(2020年版)四部通则,对九节茶水分、总灰分、酸不溶性灰分及浸出物进行检查;采用高效液相色谱法测定九节茶中异嗪皮啶和迷迭香酸的含量.结果 九节茶薄层鉴别特征斑点清晰,分离度良好;13批九节茶药材水分平均为9.4%,总灰分平均为5.2%,酸不溶性灰分平均为0.5%,浸出物平均为12.6%;异嗪皮啶平均含量为0.026%,迷迭香酸平均含量为0.030%.结论 本研究建立的薄层鉴别、检查及含量测定方法专属性强、准确度高、操作简便,可用于九节茶药材质量标准研究.
Quality standard of Sarcandra glabra
Objective To improve the quality standard of Sarcandra glabra.Methods Thirteen batches of Sarcandra glabra from different areas were used as the research object.As a control substance,isofraxidin and standard herb were identified by thin layer chromatography(TLC).The moisture,total ash,acid-insoluble ash,and extract of Sarcandra glabra were determined according to the general principles in Chinese Pharmacopoeia(2020 Edition).The content of isofraxidin and rosmarinic acid was determined by high-performance liquid chromatography(HPLC).Results The spots of TLC were clear and well separated.The content of moisture,total ash,acid-insoluble ash and extract of samples was 9.4%,5.2%,0.5%and 12.6%,respectively.The content of isofraxidin and rosmarinic acid was 0.026%and 0.030%,respectively.Conclusion This method is highly specific,accurate and simple,which may be used in the quality control of Sarcandra glabra.

Sarcandra glabraquality standardisofraxidinrosmarinic acidTLCHPLC

苏锐辉、于小庆、江涛、汤迎湛、彭红英、李怡芳、何蓉蓉

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广州白云山敬修堂药业股份有限公司,广州 510130

暨南大学药学院,广州 510632

九节茶 质量标准 异嗪皮啶 迷迭香酸 薄层色谱 高效液相色谱

国家自然科学基金-广东联合基金项目广东省中药材产业化基地建设项目

U1801284

2024

中南药学
湖南省药学会

中南药学

CSTPCD
影响因子:0.736
ISSN:1672-2981
年,卷(期):2024.22(1)
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