Establishment of fingerprint and content determination of Zhuang medicine Huosuoteng decoction
Objective To establish the HPLC fingerprints for Huosuoteng decoction and determine the content.Methods HPLC fingerprints of 10 batches of Huosuoteng decoction were obtained and the common peaks identified.CA,PCA and OPLS-DA were conducted to screen out the different substances and determine their content.Results There were 12 common peaks in the fingerprint,with similarity was greater than or equal to 0.91.Hyperoside was the 9 peak while quercitrin was the 12 peak.The CA and PCA analyses were consistent.The 10 batches of medicinal materials were divided into 3 groups.Eight substances were screened by OPLS-DA analysis.The content of hyperoside and quercitrin was 0.15～0.66 mg·g-1 and 1.71～6.52 mg·g-1,respectively,and the content of index components in S4 and S5 batches was the highest.Conclusion The HPLC fingerprints and content determination method have good stability,which can be used to provide relevant reference for the quality control and component material basis of the preparation.
万方数据
维普
