摘要
目的 研究香附Cyperi Rhizoma的化学成分及其抗炎活性.方法 利用硅胶、Sephadex LH-20 和制备型正相HPLC对香附 95%乙醇提取物进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构.以脂多糖诱导的小鼠小胶质细胞BV-2 炎症模型评价其抗炎活性.结果 从香附中分离得到15个化合物,鉴定为:24-methylenecycloartenone(1)、cyperenal(2)、patchoulenone(3)、4,5,6,7,8,8a-六氢-3,4,8,8-四甲基-1H-3a,7-亚甲基甘菊环-4-醇甲酸酯(4)、4-烯-广藿香醇(5)、cyperenol(6)、cyperolactone(7)、(-)-caryophyllene oxide(8)、humulene epoxideⅡ(9)、humulene diepoxide A(10)、α-tocopherol(11)、litseachromolaevane A(12)、hyperhubein G(13)、nootkatone(14)和mustakone(15).化合物4和11可显著抑制NO的释放,抑制率分别为(49.17±0.01)%和(51.91±0.05)%.结论 本文首次使用制备型正相HPLC技术对香附石油醚层进行系统分离,化合物 2、6、9～11 和 13为首次从莎草科植物中分离得到,化合物12为首次从莎草属植物中分离得到,化合物4、5和7为首次从香附中分离得到.化合物4 和11有较强的体外抗炎活性.
Abstract
Objective To determine the chemical constituents of Cyperi Rhizoma and their anti-inflammatory activities.Methods The 95%ethanol extract was separated and purified by silica gel,Sephadex LH-20 and preparative normal phase HPLC.The structures of the compounds were identified according to their physicochemical properties and spectral data.Their anti-inflammatory activities were evaluated by lipopolysaccharide induced BV-2 microglial cell model.Results Totally 15 compounds were isolated and identified as follows:24-methylenecycloartenone(1),cyperenal(2),patchoulenone(3),3,4,8,8-tetramethyl-4,5,6,7,8,8a-hexahydro-1H-3a,7-methanoazulen-4-ol(4),patchoulan-4-ene-6-ol(5),cyperenol(6),cyperolactone(7),(-)-caryophyllene oxide(8),humulene epoxide Ⅱ(9),humulene diepoxide A(10),α-tocopherol(11),litseachromolaevane A(12),hyperhubein G(13),nootkatone(14),and mustakone(15).Compounds 4 and 11 significantly inhibited the release of nitric oxide,and the inhibition rates were(49.17±0.01)%and(51.91±0.05)%,respectively.Conclusion The preparative normal phase HPLC has been used for the first time to systematically separate the petroleum ether layer of Cyperi Rhizoma.Compounds 2,6,9～11 and 13 have been isolated from the Cyperaceae family for the first time,compound 12 isolated from the genus Cyperus,and compounds 4,5 and 7 isolated from Cyperi Rhizoma for the first time.Compounds 4 and 11 exhibit strong in vitro anti-inflammation activities.
基金项目
国家中医药管理局中医药传承与创新"百千万"人才工程——岐黄工程首席科学家支持项目(国中医药人教函[2021]7号)
国家中医药管理局中医药传承与创新"百千万"人才工程(岐黄工程)岐黄学者支持项目(国中医药人教函[2018]284号)
2022年全国名老中医药专家传承工作室建设项目(国中医药人教函[2022]75号)
第七批全国老中医药专家学术经验继承工作项目(国中医药人教函[2022]76号)
维普
万方数据