Chemical constituents of Cyperi Rhizoma and their anti-inflammation activities
Objective To determine the chemical constituents of Cyperi Rhizoma and their anti-inflammatory activities.Methods The 95%ethanol extract was separated and purified by silica gel,Sephadex LH-20 and preparative normal phase HPLC.The structures of the compounds were identified according to their physicochemical properties and spectral data.Their anti-inflammatory activities were evaluated by lipopolysaccharide induced BV-2 microglial cell model.Results Totally 15 compounds were isolated and identified as follows:24-methylenecycloartenone(1),cyperenal(2),patchoulenone(3),3,4,8,8-tetramethyl-4,5,6,7,8,8a-hexahydro-1H-3a,7-methanoazulen-4-ol(4),patchoulan-4-ene-6-ol(5),cyperenol(6),cyperolactone(7),(-)-caryophyllene oxide(8),humulene epoxide Ⅱ(9),humulene diepoxide A(10),α-tocopherol(11),litseachromolaevane A(12),hyperhubein G(13),nootkatone(14),and mustakone(15).Compounds 4 and 11 significantly inhibited the release of nitric oxide,and the inhibition rates were(49.17±0.01)%and(51.91±0.05)%,respectively.Conclusion The preparative normal phase HPLC has been used for the first time to systematically separate the petroleum ether layer of Cyperi Rhizoma.Compounds 2,6,9～11 and 13 have been isolated from the Cyperaceae family for the first time,compound 12 isolated from the genus Cyperus,and compounds 4,5 and 7 isolated from Cyperi Rhizoma for the first time.Compounds 4 and 11 exhibit strong in vitro anti-inflammation activities.
Cyperi Rhizomachemical constituentisolation and identificationanti-inflammation activity
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维普
