Mechanism of Ginseng-Chemotaxis intervention in COPD based on the serum metabolomics
Objective To determine the intervention role of Ginseng-Chemotaxis in chronic obstructive pulmonary disease(COPD)based on serum metabolomics.Methods Totally 24 male SD rats were randomly divided into a control group,a model group,an aminophylline group,and a Ginseng-Chemotaxis group.Except for the control group,the other rats were induced by lipopolysaccharide(LPS)and smoke stimulation to construct a COPD model.After the modeling,the control group and the model group were given equal doses of normal saline.The Ginseng-Chemotaxis group(0.027 g·mL-1)and the aminophylline group(0.009 g·mL-1)were administered at 1 mL/100 g for 15 d.HE staining was used to observe the morphological changes in the lung tissue and the airway.ELISA was used to detect the serum levels of TNF-α,IL-1β,MMP-9,and TIMP-1.UPLC-Q-TOF/MS was applied to analyze the serum metabolism of the model group and the Ginseng-Chemotaxis group,to screen the potential biomarkers and their related pathways,and verify the related pathways by Western blot.Results The Ginseng-Chemotaxis group significantly improved the inflammatory infiltration of the lung tissues,thickened the airway wall,and reduced the levels of inflammatory factors TNF-α,IL-1β,MMP-9 and TIMP-1 in the serum(P＜0.05,P＜0.01).Serum metabolomics analysis identified 33 potential biomarkers,involving histidine metabolism,arginine synthesis,arachidonic acid metabolism,and glycerophospholipid metabolism.After validation by Western blot,the treatment promoted arginine synthesis and inhibited arachidonic acid metabolism.Conclusion The combination of Ginseng-Chemotaxis may improve inflammation in the lung tissue and thicken the airway wall in rats with COPD,possibly through promoting arginine synthesis and inhibiting arachidonic acid metabolism.
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