Effect and mechanism of longan aril polysaccharides on phagocytosis of Aβ-induced tolerant microglias
Objective To determine the effect and mechanism of longan aril polysaccharides(LAPs)on phagocytosis in Aβ-induced tolerant microglia(BV2).Methods Cells were treated with 4 μmol·L-1 of Aβ for(48 h+24 h)to establish an tolerant model,while the LAPs group was intervened by 0.8 and 1.6 mg·mL-1 LAPs.MTT method was used to detect the cell proliferation level.JC-1 kit was used to detect mitochondrial membrane potential.Inverted fluorescence microscope was used to observe the phagocytosis function of the cells and the effect of mTOR inhibitor(Rapa)and HIF-1α inhibitor(BAY)on the action of LAPs.Western blot was used to detect the protein expression of PI3K,Akt,p-Akt and HIF-1α.Results The proliferation rate and target protein expression of BV2 cells treated with Aβ were increased,and the mitochondrial membrane potential and cytophagocytosis were decreased(P＜0.05,P＜0.01).LAPs reversed these indexes,and inhibitors blocked their roles in improving the phagocytic function(P＜0.05,P＜0.01).Conclusion LAPs can improve the phagocytosis of Aβ-induced tolerant BV2 cells,and the pathway of action may be related to the enhancement of the proliferation capacity and the level of mitochondrial membrane potential of tolerant BV2 cells,and may involve the activation of the PI3K/Akt/mTOR/HIF-1α signalling pathway.
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