UPLC-Q-TOF-MS/MS for rapid identification of chemical components in Jiawei Bazhen Yimu ointment
Objective To analyze the chemical compositions in Jiawei Bazhen Yimu ointment by UPLC-Q-TOF-MS/MS to control the quality of Jiawei Bazhen Yimu ointment.Methods An Agilent Poroshell SB-C18(100 mm×4.6 mm,2.7 μm)chromatographic column was used with mobile phase of 0.2%formic acid solution(A)-methanol(B)under positive ion mode and water(A)-methanol(B)under negative ion mode.The flow rate was 0.4 mL·min-1 and the column temperature was 30℃.Mass spectrometric data were obtained under electrospray inoization(ESI)in both positive and negative ion modes.Combined with literature retrieval and comparison of reference substances,the chemical components in Jiawei Bazhen Yimu ointment were analyzed.Results Totally 140 compounds were identified,including 48 organic acid and their derivatives,32 flavonoids ingredients,4 alkaloids components,10 sugar and glycosidic ingredients,19 terpenoids,and 27 other ingredients.Conclusion The main chemical components in Jiawei Bazhen Yimu ointment are identified,laying the foundation for its quality control.
万方数据
维普
