Fingerprints of Mashi Qingfei mixture and content determination of 7 components
Objective To establish the fingerprints of Mashi Qingfei mixture and determine 7 components such as ephedrine hydrochloride.Methods The fingerprints of 12 batches of Maishi Qingfei mixture were established by HPLC and its similarity was evaluated.Different components were screened by chemical pattern recognition,and the content of 7 components such as ephedrine hydrochloride was determined.Results Totally 16 common peaks were identified,and the similarity was higher than 0.900.Meanwhile 7 components were identified.The 12 batches of samples were divided into four categories,and peak 8,7(amygdalin),3(ephedrine hydrochloride),9,10(liquiritin),4(pseudoephedrine hydrochloride),and 6 were screened as the differential components.The contents of ephedrine hydrochloride,pseudoephedrine hydrochloride,amygdalin,liquiritin,glycyrrhizic acid,honokiol,magnoliol in the 12 batches of Mashi Qingfei mixture were 29.620～156.274,12.504～85.355,245.089～643.440,20.357～79.625,35.117～108.893,0.310～5.556,and 1.777～10.382 μg·mL-1,respectively.Conclusion The fingerprint and content determination method can comprehensively evaluate the quality of Mashi Qingfei mixture.
Mashi Qingfei mixturefingerprintchemical pattern recognitionhigh performance liquid chromatographyquality evaluation
万方数据
维普
